Methods to monitor classical protein-tyrosine phosphatase oxidation

Dominant role of the protein-tyrosine phosphatase CD148 in regulating platelet activation relative to protein-tyrosine phosphatase-1B.

OBJECTIVE The receptor-like protein-tyrosine phosphatase (PTP) CD148 and the nontransmembrane PTP1-B have been shown to be net positive regulators of Src family kinases in platelets. In the present study, we compared the relative contributions of these PTPs in platelet activation by the major glycoprotein, glycoprotein VI, α(IIb)β(3), and C-type lectin-like receptor 2 (CLEC-2). METHODS AND RE...

Singlet oxygen inactivates protein tyrosine phosphatase-1B by oxidation of the active site cysteine.

Singlet oxygen ((1)O(2)), an electronically excited form of molecular oxygen, is a mediator of biological effects of ultraviolet A radiation, stimulating signaling cascades in human cells. We demonstrate here that (1)O(2) generated by photosensitization or by thermodecomposition of 3,3'-(1,4-naphthylidene)dipropionate-1,4-endoperoxide inactivates isolated protein tyrosine phosphatases (PTPases)...

Developing new methods to monitor phase II fuzzy linear profiles

In some quality control applications, the quality of a process or a product is described by the relationship between a response variable and one or more explanatory variables, called a profile. Moreover, in most practical applications, the qualitative characteristic of a product/service is vague, uncertain and linguistic and cannot be precisely stated. The purpose of this paper is to propose a ...

Chemical rescue of protein tyrosine phosphatase activity.

We report that the activity of a rationally engineered protein tyrosine phosphatase (PTP) mutant can be fully rescued by the addition of the biarsenical fluorescein derivative FlAsH, a compound that does not affect the activity of wild-type PTPs.

The Protein Tyrosine Phosphatase SHP-1